Aberrant Heterozygotes in Escherichia Coli

Bactericidal action of streptomycin and comparison with spectinomycin in heterozygotes of Escherichia coli.

Str(s)/str(r) heterozygotes of Escherichia coli K-12 are shown to be sensitive to the lethal as well as the inhibitory action of streptomycin. The rate of killing was lower in heterozygotes than in sensitive homozygotes, and among heterozygotes it was lower in those with a higher proportion of resistant ribosomes. These strains also differed, in a parallel manner, in the kinetics of inhibition ...

Functional motifs in Escherichia coli NC101

Escherichia coli (E. coli) bacteria can damage DNA of the gut lining cells and may encourage the development of colon cancer according to recent reports. Genetic switches are specific sequence motifs and many of them are drug targets. It is interesting to know motifs and their location in sequences. At the present study, Gibbs sampler algorithm was used in order to predict and find functional m...

Advances and challenges in recombinant protein expression in Escherichia coli

Aberrant cell division and random FtsZ ring positioning in Escherichia coli cpxA* mutants.

In Escherichia coli, certain mutations in the cpxA gene (encoding a sensor kinase of a two-component signal transduction system) randomize the location of FtsZ ring assembly and dramatically affect cell division. However, deletion of the cpxRA operon, encoding the sensor kinase and its cognate regulator CpxR, has no effect on division site biogenesis. It appears that certain mutant sensor kinas...

The Effect of Aberrant Protein Synthesis on the Production of Capsular Polysaccharide in Escherichia coli B23

Streptomycin, an aminoglycoside, binds to and modifies prokaryotic ribosomes leading to a decrease in protein synthesis and the production of mistranslated proteins. Previous studies reported that Escherichia coli B23, under sub-lethal concentrations of streptomycin, exhibited an increase in capsule polysaccharide (CPS). The mechanism through which this increase in CPS occurs was unknown. In th...